Single-celled organisms turn sand green
by Judith Lea Garfield
Mar 21, 2009 | 4917 views | 1 1 comments | 12 12 recommendations | email to a friend | print
If green acres is the place you want to see, stroll the beach at low tide first thing in the morning.                                                                            © 2009 JUDITH LEA GARFIELD
If green acres is the place you want to see, stroll the beach at low tide first thing in the morning. © 2009 JUDITH LEA GARFIELD
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© 2009 JUDITH LEA GARFIELD
An errant piece of kelp demonstrates vertical migration of Euglena. The green microbes unblocked by the kelp frond remain on the surface, taking advantage of sunlight to produce food for themselves the old-fashioned way: photosynthesis.
© 2009 JUDITH LEA GARFIELD An errant piece of kelp demonstrates vertical migration of Euglena. The green microbes unblocked by the kelp frond remain on the surface, taking advantage of sunlight to produce food for themselves the old-fashioned way: photosynthesis.
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© 2009 JUDITH LEA GARFIELD
Euglena cells under the kelp are blocked from the sun so they have descended into the sand to look for nutrition the new-fangled, evolutionary-forward way, by engulfing and digesting nutrient particles.
© 2009 JUDITH LEA GARFIELD Euglena cells under the kelp are blocked from the sun so they have descended into the sand to look for nutrition the new-fangled, evolutionary-forward way, by engulfing and digesting nutrient particles.
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COURTESY MICHAEL KINGSTON, Ph.D.
At about 50 microns, teardrop-shaped Euglena cells are photographed against their sand background. The green part is the chloroplast, while the red spot closer to the other end is the “shade” spot.
COURTESY MICHAEL KINGSTON, Ph.D. At about 50 microns, teardrop-shaped Euglena cells are photographed against their sand background. The green part is the chloroplast, while the red spot closer to the other end is the “shade” spot.
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Those of us who regularly ply the beach at La Jolla Shores may wonder what the patches or swaths of green sand are all about. Not a St. Patrick’s Day stunt gone permanently awry, the verdant coating is made up of billions and billions of microorganisms belonging to the genus Euglena. A member of the protista, a catchall kingdom for organisms that aren’t animals, plants or fungi, the single-celled Euglena is a freshwater organism oddly found in a saltwater environment. It manages to survive by tolerating brackish conditions (wherein freshwater runoff mixes with ocean water) like those found at the Shores.

The green presentation appears compliments of the cell’s chloroplast, the same organelle found in plants and known as the root, so to speak, of photosynthesis, the method of garnering food from sunlight. Not all Euglena species photosynthesize, but the ones that do trace their origins to an auspicious interaction with a single-celled alga. Simply put, a Euglena engulfed the alga and, instead of digesting the foreign body, somehow incorporated and used it as another avenue for gaining nutrition. I say another because a Euglena can engulf nutrient particles in the sand for nutrition as well. Since a Euglena cell reproduces by splitting in two (fission), only one Euglena cell and its permanent green guest would have been required to populate this new, evolutionary line.

A Euglena enjoys a peripatetic lifestyle thanks to its whiplike tail, the flagellum, which is anchored into the cell’s polar end. Being able-bodied is key because a Euglena’s life is closely connected to tidal ebb and flow. Unlike the tides, though, a Euglena’s rhythm is vertical through the sand substrate. During daytime, when low tide exposes sand to air, millions of Euglena cells head to the sand’s surface to absorb the sun’s rays. About a half hour before the first wave from an incoming tide sweeps across the sand, a portion of a Euglena population begins migrating downward so the green is less intense. The many lollygagger Euglena cells caught on the surface are washed higher onto the beach. As the wave retreats, the cells now transported higher up the beach percolate into the sand.

Michael Kingston, environmental biologist at Elon University, studied Euglena viridis, the species found at La Jolla Shores, and discovered that when it comes to sunlight, like dessert, too much of a good thing isn’t better.

Kingston said, “We found that really bright sunlight inhibits photosynthesis. The highest photosynthetic rate is found at about 60 percent sunlight. Below about 4 millimeters [in the sand] there is no light, so the cells adjust to the strength of the sunlight by photosynthesizing from the surface to three millimeters.”

Not surprisingly, then, a Euglena’s movement in sand and its photosynthetic machinery are intimately linked. Within a Euglena’s body lies a circular, red body called a light spot, or even less accurately an eye spot, that faces one side of the cell. Both names are misnomers because the red spot actually shades the light. So really, it’s a shade spot. A light-detecting organ exists as well but it’s near the base of the flagellum. The shade spot and the light organ partner to influence whether a Euglena moves toward the light or away from it. As the whiplike tail propels the Euglena forward, the cell spins in rotation, not unlike the way a thrown football travels from point A to point B while simultaneously spinning. Because a Euglena cell is constantly rotating, the shade spot alternates being struck by light and shaded from light. Incoming information from the periodic, split-second shading determines whether Euglena angles toward or away from the light.

When there’s not enough light to photosynthesize, a Euglena population moves deeper into the sand to search out nutrient particles.

Kingston said, “Euglena is typically found where there is higher organic output, such as runoff locations where organic compounds such as those from fertilizers [ammonia nitrates, et cetera] and sewage wash into the ocean. In California, I look for cliffs and runoff to find Euglena. It is the seepage of freshwater and its extra nutrient content moving onto the beach and into the ocean that make for an ideal Euglena habitat.”

These conditions have Kingston dreaming that in the future Euglena cells may be used like canaries in a coal mine to track groundwater flow. Know that pollution doesn’t only wash from the sand’s surface into the ocean but also seeps up from under the sand and makes its way into the ocean. Freshwater from the tide washing in and out may dilute low-level, nonpoint sources of pollution so they don’t show up on a seepage meter. Employing a Euglena assay may better indicate pollution before it fouls the ocean because low concentrations of enriched freshwater are all that’s necessary for a Euglena population to bloom. This contrasts with larger-scale flows from rain runoff events needed by seepage meters to read pollution levels. Consequently, measuring Euglena concentrations may have merit as a superior way to monitor water quality. So once again, it’s good to be green.

— Judith Lea Garfield, biologist and underwater photographer, has authored two natural history books about the underwater park off La Jolla Cove and La Jolla Shores. www.judith.garfield.org. Questions, comments or suggestions? Email jgarfield@ucsd.edu.
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metalworksguy
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April 25, 2011
Look what it did to this thing!!

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